Coding

Part:BBa_K4644004:Design

Designed by: Han Cao   Group: iGEM23_BUCT   (2023-09-23)


pgsBCAE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 737
    Illegal XbaI site found at 1032
    Illegal PstI site found at 382
    Illegal PstI site found at 1013
    Illegal PstI site found at 2028
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 737
    Illegal PstI site found at 382
    Illegal PstI site found at 1013
    Illegal PstI site found at 2028
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 737
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 737
    Illegal XbaI site found at 1032
    Illegal PstI site found at 382
    Illegal PstI site found at 1013
    Illegal PstI site found at 2028
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 737
    Illegal XbaI site found at 1032
    Illegal PstI site found at 382
    Illegal PstI site found at 1013
    Illegal PstI site found at 2028
    Illegal NgoMIV site found at 1277
    Illegal AgeI site found at 2968
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We constructed PETlac-pgsBCAE plasmid

Source

Providencia rettgeri strain JNB815.Sequence found from Genbank https://www.ncbi.nlm.nih.gov/nuccore/NZ_CP029736.1?report=fasta&from=1655614&to=1655937&strand=true


References

Bai, N.; He, Y.; Zhang, H.; Zheng, X.; Zeng, R.; Li, Y.; Li, S.; Lv, W. γ-Polyglutamic Acid Production, Biocontrol, and Stress Tolerance: Multifunction of Bacillus subtilis A-5 and the Complete Genome Analysis. Int. J. Environ. Res. Public Health 2022, 19, 7630.

Xiaoyu Wei, Zhen Chen, Ailing Liu, Lijie Yang, Yiyuan Xu, Mingfeng Cao, Ning He,Advanced strategies for metabolic engineering of Bacillus to produce extracellular polymeric substances,Biotechnology Advances,Volume 67,2023,108199,ISSN 0734-9750,